Antigen

Antigen Class

TFs and others

Antigen

Rad21

Cell type

Cell type Class

Neural

Cell type

Cerebellum

MeSH Description

The part of brain that lies behind the BRAIN STEM in the posterior base of skull (CRANIAL FOSSA, POSTERIOR). It is also known as the "little brain" with convolutions similar to those of CEREBRAL CORTEX, inner white matter, and deep cerebellar nuclei. Its function is to coordinate voluntary movements, maintain balance, and learn motor skills.

Sample

source_name

Anterior Mouse Cerebellum

tissue

Anterior Mouse Cerebellum

genotype

CHD7 cKO

Sex

pooled male and female

strain

C57BL/6

chip antibody

RAD21 (Abcam, #ab992)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

RNA was extracted from the anterior cerebella of P4 mice using Trizol (Invitrogen) according to the manufacturer's instructions. ChIP lysates clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

21699710

Reads aligned (%)

100.0

Duplicates removed (%)

5.2

Number of peaks

12365 (qval < 1E-05)

mm9

Number of total reads

21699710

Reads aligned (%)

100.0

Duplicates removed (%)

5.2

Number of peaks

12405 (qval < 1E-05)